NRAS Mutation Analysis Kit
|Product Name/Description||No. of Reactions*||Product Code|
|NRAS Mutation Detection Kit||50||NRAS-RT50|
|RAS c.59/117 Mutation Detection Kit †||50||RASX-RT50|
* Includes all controls.
† For detection of KRAS c.59, NRAS c.59/117 somatic mutations. Available with purchase of NRAS-RT50, KRAS-RT50, or KRBR-RT50.
NRAS Mutations and Cancer
NRAS is a member of the RAS family of GTPases and plays a central role in the MAPK signaling pathway. Activating mutations in exon 2 (codon 12/13), exon 3 (codon 61), and exon 4 (codon 146) have been found in various cancers, including melanoma (13-25%), colorectal cancer (1-6%), lung cancer (1%), hepatocellular carcinoma (10%), myeloid leukemias (14%), and thyroid carcinoma (7%).
The table below lists the mutations detected by the NRAS Mutation Detection kit.
|Exon||Mutation Name||Nucleotide Change||Amino Acid Change|
|2||G12C||c.34G>T||Glycine (G) to Cystein (C)|
|G12D||c.35G>A||Glycine (G) to Aspartic acid (D)|
|G12S||c.34G>A||Glycine (G) to Serine (S)|
|G13V||c.38G>T||Glycine (G) to Valine (V)|
|G13R||c.37G>C||Glycine (G) to Arginine (R)|
|3||Q61K||c.181C>A||Glutamine (Q) to Lysine (K)|
|Q61R||c.182A>G||Glutamine (Q) to Arginine (R)|
|Q61L||c.182A>T||Glutamine (Q) to Leucine (L)|
|Q61H||c.183A>C & c.183A>T||Glutamine (Q) to Histidine (H)|
|4||A146T||c.436G>A||Alanine (A) to Threonine (T)|
For expanded coverage, the RAS c.59/117 Mutation Detection Kit detects KRAS c.59, NRAS c.59/117 somatic mutations.
EntroGen’s NRAS mutation detection kit is a polymerase chain reaction (PCR)-based assay that uses allele-specific primers to identify the presence of the mutations listed above. The testing procedure involves three (3) simple steps:
- Isolation of DNA from tumor biopsies, paraffin-embedded sections (FFPE), or fresh frozen tumors
- Amplification of regions of the NRAS gene using allele-specific primers.
- Detection of amplification product on a Real-Time PCR instrument.
This test can be completed in approximately 2 hours from isolation of DNA to test result.
Equipment and Materials
EntroGen’s NRAS mutation detection kit requires a real-time PCR instrument capable of detecting FAM and VIC fluorescent probes.
This test includes reagents required for PCR amplification/detection, as well as validated reaction controls. Columns and reagents for DNA isolation are not included.
EntroGen’s NRAS mutation analysis kit is provided for research use only (RUO). Not for use in diagnostic procedures.
EntroGen’s NRAS mutation analysis kit is available for research (RUO) and diagnostic (CE-IVD) purposes.