NRAS Mutation Analysis Kit
Available Products
| Product Name/Description | No. of Reactions* | Product Code |
| NRAS Mutation Detection Kit | 50 | NRAS-RT50 |
| RAS c.59/117 Mutation Detection Kit † | 50 | RASX-RT50 |
* Includes all controls.
† For detection of KRAS c.59, NRAS c.59/117 somatic mutations. Available with purchase of NRAS-RT50, KRAS-RT50, or KRBR-RT50.
NRAS Mutations and Cancer
NRAS is a member of the RAS family of GTPases and plays a central role in the MAPK signaling pathway. Activating mutations in exon 2 (codon 12/13), exon 3 (codon 61), and exon 4 (codon 146) have been found in various cancers, including melanoma (13-25%), colorectal cancer (1-6%), lung cancer (1%), hepatocellular carcinoma (10%), myeloid leukemias (14%), and thyroid carcinoma (7%).
The table below lists the mutations detected by the NRAS Mutation Detection kit.
| Exon | Mutation Name | Nucleotide Change | Amino Acid Change |
| 2 | G12C | c.34G>T | Glycine (G) to Cystein (C) |
| G12D | c.35G>A | Glycine (G) to Aspartic acid (D) | |
| G12S | c.34G>A | Glycine (G) to Serine (S) | |
| G13V | c.38G>T | Glycine (G) to Valine (V) | |
| G13R | c.37G>C | Glycine (G) to Arginine (R) | |
| 3 | Q61K | c.181C>A | Glutamine (Q) to Lysine (K) |
| Q61R | c.182A>G | Glutamine (Q) to Arginine (R) | |
| Q61L | c.182A>T | Glutamine (Q) to Leucine (L) | |
| Q61H | c.183A>C & c.183A>T | Glutamine (Q) to Histidine (H) | |
| 4 | A146T | c.436G>A | Alanine (A) to Threonine (T) |
For expanded coverage, the RAS c.59/117 Mutation Detection Kit detects KRAS c.59, NRAS c.59/117 somatic mutations.
EntroGen’s NRAS mutation detection kit is a polymerase chain reaction (PCR)-based assay that uses allele-specific primers to identify the presence of the mutations listed above. The testing procedure involves three (3) simple steps:
- Isolation of DNA from tumor biopsies, paraffin-embedded sections (FFPE), or fresh frozen tumors
- Amplification of regions of the NRAS gene using allele-specific primers.
- Detection of amplification product on a Real-Time PCR instrument.
This test can be completed in approximately 2 hours from isolation of DNA to test result.
Equipment and Materials
EntroGen’s NRAS mutation detection kit requires a real-time PCR instrument capable of detecting FAM and VIC fluorescent probes.
This test includes reagents required for PCR amplification/detection, as well as validated reaction controls. Columns and reagents for DNA isolation are not included.
Intended Use
USA:
EntroGen’s NRAS mutation analysis kit is provided for research use only (RUO). Not for use in diagnostic procedures.
Europe:
EntroGen’s NRAS mutation analysis kit is available for research (RUO) and diagnostic (CE-IVD) purposes.