GIST Mutation Screening Panel
Available Products
| Product Name/Description | No. of Reactions* | Product Code |
| GIST Mutation Screening Panel for Real-Time PCR | 44 | GIST-RT44 |
*Includes all controls.
PDGFRA & c-KIT Mutations in GIST
Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal tumors of the GI tract. Over 80% of GISTs harbor activating mutation in c-KIT and approximately 5-7% in PDGFRA. Although these mutations cause tumorigenesis, they also predict response to targeted therapies and provide prognostic information.The GIST Mutation Screening Panel detects the following mutations:
| Gene | Mutation |
| KIT | EX9_p.Y503_F504insAY |
| KIT | EX11_ p.V559D |
| KIT | EX11_del |
| KIT | EX11_p.L576P |
| KIT | EX11_p.V559A |
| KIT | EX11_p.V559G |
| KIT | EX11_p.V560D |
| KIT | EX11_p.W557G |
| KIT | EX11_W557R |
| KIT | EX13_p.K642E |
| KIT | EX17_p.D816H |
| KIT | EX17_p.D816V |
| KIT | EX17_p.D820G |
| KIT | EX17_p.D820Y |
| KIT | EX17_p.D820Y |
| KIT | EX17_p.K818R |
| KIT | EX17_p.N822H |
| KIT | EX17_p.N822K |
| KIT | EX17_p.Y823D |
| PDGFRA | EX18_p.D842V |
Testing Procedure and Analysis
EntroGen’s GIST mutation screening panel is a polymerase chain reaction (PCR)-based assay that uses allele-specific primers to identify the presence of somatic mutations in c-KIT and PDGFRA genes. The testing procedure involves three (3) simple steps:
- Isolation of DNA from tumor biopsies, paraffin-embedded sections (FFPE), or fresh frozen tumors
- Amplification of regions of the c-KIT and PDGFRA genes using allele-specific primers
- Analysis of the captured amplification data.
This test can be completed in approximately 2 hours from isolation of DNA to test result.
Equipment and Materials
EntroGen’s GIST mutation screening panel requires a real-time PCR instrument capable of detecting FAM, VIC, ROX and CY5 fluorescent probes.
This test includes reagents required for the PCR amplification/detection, as well as validated reaction controls. Columns and reagents for DNA isolation are not included.
Intended Use
USA: Available for research use only (RUO). Not for use in diagnostic procedures.
Europe: Available for research (RUO) and diagnostic (CE-IVD) purposes.